Programmed factor binding to simian virus 40 GC-box replication and transcription control sequences.

AUTOR(ES)

Buchanan, R L

RESUMO

Nuclear footprinting revealed a temporal program involving factor binding to the repetitive GC-box DNA elements present in the simian virus 40 regulatory region. This program specified ordered and directional binding to these tandem regulatory sequences in vivo during the late phase of infection. The program was interrupted by the DNA replication inhibitor aphidicolin or by inactivation of the viral replication factor simian virus 40 T antigen, suggesting a link between viral DNA replication and new factor binding. Measurements of DNA accumulation in viruses lacking either the distal or proximal halves of the GC-box region suggested that the region has a dual role in replication control. Overall, the data point to important relationships between DNA replication and factor binding to the GC-box DNA, a multifunctional regulatory region.

Documentos Relacionados

• Factor interactions at simian virus 40 GC-box promoter elements in intact nuclei.
• A GC-box motif upstream of the B19 parvovirus unique promoter is important for in vitro transcription.
• Transcriptional initiation is controlled by upstream GC-box interactions in a TATAA-less promoter.
• Cloning and Characterization of a GC-Box Binding Protein, G10BP-1, Responsible for Repression of the Rat Fibronectin Gene
• Polyomavirus and simian virus 40 large T antigens bind to common DNA sequences.