Promoters recognized by Escherichia coli RNA polymerase selected by function: highly efficient promoters from bacteriophage T5.
AUTOR(ES)
Gentz, R
RESUMO
Highly efficient promoters of coliphage T5 were identified by selecting for functional properties. Eleven such promoters belonging to all three expression classes of the phage were analyzed. Their average AT content was 75% and reached 83% in subregions of the sequences. Besides the well-known conserved sequences around -10 and -33, they exhibited homologies outside the region commonly considered to be essential for promoter function. Interestingly, the consensus hexamers around -10 (TAT AAT) and -35 (TTG ACA) were never found simultaneously within the sequence of highly efficient promoters. Several of these promoters compete extremely well for Escherichia coli RNA polymerase and can be used for the efficient in vitro synthesis of defined RNA species. In addition, some of these promoters accept 7-mGpppA as the starting dinucleotide, thus producing capped mRNA in vitro which can be utilized in various eucaryotic translation systems.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=214212Documentos Relacionados
- Modification of RNA polymerase from Escherichia coli by pre-early gene products of bacteriophage T5.
- Membrane damage in abortive infections of colicin Ib-containing Escherichia coli by bacteriophage T5.
- Early events after infection of Escherichia coli by bacteriophage T5. II. Control of the bacteriophage-induced 5'-nucleotidase activity.
- Amino acid and sugar transport in Escherichia coli (ColIb) during abortive infection by bacteriophage T5.
- Early events after infection of Escherichia coli by bacteriophage T5. III. Inhibition of uracil-DNA glycosylase activity.