Properties of a Soluble DNA Polymerase Isolated from Rous Sarcoma Virus
AUTOR(ES)
Duesberg, Peter
RESUMO
The DNA polymerase of the Prague strain of Rous sarcoma virus of subgroup C and of the Schmidt-Ruppin strain of subgroup A has been solubilized. DNA polymerase purified by sucrose gradient sedimentation and chromatography on DEAE-cellulose represented less than 2% of the soluble [14C]protein of the virus. The enzyme was separated from 90% of the viral glycoprotein; it is probably different from the viral group-specific antigen. The sedimentation coefficient (s20, w) of the soluble DNA polymerase was 8 S before, and 6 S after, incubation with pancreatic RNase. The molecular weight of the 8S DNA polymerase was estimated to be about 170,000, and that of the 6S DNA polymerase to be about 110,000.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=389034Documentos Relacionados
- Comparative Properties of RNA and DNA Templates for the DNA Polymerase of Rous Sarcoma Virus
- Properties of a Ribonucleoprotein Particle Isolated from Nonidet P-40-Treated Rous Sarcoma Virus
- HOMOLOGY BETWEEN RNA FROM ROUS SARCOMA VIRUS AND DNA FROM ROUS SARCOMA VIRUS-INFECTED CELLS*
- Physical properties of Rous Sarcoma Virus RNA.
- Rescue of Rous Sarcoma Virus from Rous Sarcoma Virus-Transformed Mammalian Cells 1
