Protection of Measles Virus by Sulfate Ions Against Thermal Inactivation
AUTOR(ES)
Rapp, Fred
RESUMO
Rapp, Fred (Baylor University College of Medicine, Houston, Tex.), Janet S. Butel, and Craig Wallis. Protection of measles virus by sulfate ions against thermal inactivation. J. Bacteriol. 90:132–135. 1965.—The infectivity of measles virus in water is rapidly destroyed at temperatures of 37 C and above. More than 50% of the infectivity is lost after 1 hr at 25 C, and almost 90% loss of infectivity occurs within 24 hr at 4 C. Magnesium chloride enhances the inactivation of the virus at all temperatures tested. Addition of either magnesium or sodium sulfate protects the virus against thermal inactivation. The stabilizing effect is demonstrable at temperatures ranging from 4 to 56 C, but is especially pronounced through 45 C. Prolonged storage (up to 6 weeks) of the virulent virus at 4 C in 1 m magnesium sulfate permits retention of substantial infectivity, whereas storage at 4 C in either water or 1 m magnesium chloride results in a loss of infectivity approximating 99% after 2 weeks. Magnesium chloride also enhances inactivation of the attenuated vaccine strain of measles virus. The attenuated virus, however, is strongly protected by magnesium sulfate against thermal inactivation, and retention of infectivity for long periods of time at 4 C seems feasible when the virus is kept in 1 m magnesium sulfate.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=315604Documentos Relacionados
- Protection of avian myeloblastosis virus (AMV) DNA polymerase by substrates against heat inactivation.
- The glutamyl-tRNA synthetase of Escherichia coli: substrate-induced protection against its thermal inactivation.
- Protection by Vaccination Against Pseudomonas Infection After Thermal Injury
- Protection against lethal measles virus infection in mice by immune-stimulating complexes containing the hemagglutinin or fusion protein.
- Thermal inactivation of bovine immunodeficiency virus.