Protection of Sediment-Adsorbed Transforming DNA Against Enzymatic Inactivation

AUTOR(ES)

Aardema, Barend W.

RESUMO

The action of DNase I on transforming DNA, both adsorbed to marine sediment and in solution, was investigated. DNase I reduced the transformation frequencies of free DNA more than of adsorbed DNA. Changes in salt concentration or pH did not have a significant influence on the DNA-sediment complex. Soil components other than organic materials and clay minerals can bind DNA and retard its enzymatic degradation.

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