Protein Chain Initiation by Methionyl-tRNA in Wheat Embryo*

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RESUMO

The mechanism of protein chain initiation has been investigated in a cell-free amino acid incorporation system from wheat embryos dependent on tobacco mosaic virus RNA. Analysis of the N-termini of the labeled peptide products of short-term incubations showed the presence of unblocked methionine. In addition, methionyl-tRNA (Met-tRNA) could be bound to ribosomes at 1.3 mM Mg++ in a reaction requiring viral RNA, ATP, GTP, and soluble protein factors.

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