Protein–DNA interaction mapping using genomic tiling path microarrays in Drosophila

AUTOR(ES)

Sun, Ling V.

FONTE

National Academy of Sciences

RESUMO

We demonstrate the use of a chromosomal walk (or “tiling path”) printed as DNA microarrays for mapping protein–DNA interactions across large regions of contiguous genomic DNA in Drosophila melanogaster. Microarrays were constructed with genomic DNA fragments 430–920 bp in length, covering 2.9 million base pairs of the Adh–cactus region of chromosome 2 and 85,000 base pairs of the 82F region of chromosome 3. We performed DNA localization mapping for the heterochromatin protein HP1 and for the sequence-specific GAGA transcription factor, producing a comprehensive, high-resolution map of in vivo protein–DNA interactions throughout these regions of the Drosophila genome.

Documentos Relacionados

• Native genomic blotting: high-resolution mapping of DNase I-hypersensitive sites and protein-DNA interactions.
• Genomic footprinting of the hsp70 and histone H3 promoters in Drosophila embryos reveals novel protein-DNA interactions.
• Potentiation of a polyadenylylation site by a downstream protein-DNA interaction.
• Temperature-sensitive protein–DNA dimerizers
• Detection of protein–DNA interaction with a DNA probe: distinction between single-strand and double-strand DNA–protein interaction