Protein Synthesis by Isolated Etioplasts and Chloroplasts from Pea and Wheat and the Effects of Chloramphenicol and Cycloheximide 1

AUTOR(ES)

Reger, Bonnie J.

RESUMO

Etioplasts capable of incorporating 14C-leucine into protein have been isolated from dark-grown pea and wheat plants. The requirements for leucine incorporation for etioplasts were similar to those for chloroplasts. An ATP-generating system, Mg2+, and GTP were required. The amino-acid-incorporation activity of etioplasts from wheat was comparable to that of chloroplasts on an RNA basis, whereas the activity of pea etioplasts was about 50% of the activity of pea chloroplasts. The incorporation of leucine into protein by etioplasts and chloroplasts from pea and wheat was inhibited by chloramphenicol, and to a slight extent by cycloheximide.

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