Protein synthesis in bacteriophage ghost-infected cells.

AUTOR(ES)

Takeishi, K

RESUMO

Escherichia coli B infected with T4 phage ghosts at 10 mM Mg2+ regains its protein synthesizing activity upon addition of ATP, GTP, and their generator to approximately 2% of the intact exponentially growing cells. In contrast to amino acid incorporation by intact cells, this system is sensitive to EDTA or low Mg2+. On the other hand, this system, differing from the regular cell-free system, does not respond to addition of soluble protein and ribonuclease. The ghost-infected cells were able to synthesize beta-galactosidase upon addition of the inducer isopropyl thiogalactoside. The initial rate of the induction was 2.6% of intact cells. For this induction, the addition of cyclic AMP, amino acids, ATP, GTP, UTP, CTP, and their generator was necessary. The induction of beta-galactosidase in these ghost-infected cells was very sensitive to the addition of EDTA, CaCl2, sulfhydryl blocking reagent, rifampin and chloramphenicol but insensitive to DNA synthesis inhibitors such as nalidixic acid and DNase.

Documentos Relacionados

• Metabolism of T4 Bacteriophage Ghost-Infected Cells II. Do Ghosts Cause a Generalized Permeability Change?
• Metabolism of T4 Bacteriophage Ghost-Infected Cells: Effect of Bacteriophage and Ghosts on the Uptake of Carbohydrates in Escherichia coli B
• Does Phospholipase Have a Role in Killing and Sodium Dodecyl Sulfate Lysis of T4 Ghost-Infected Escherichia coli?
• Effect of Bacteriophage Ghost Infection on Protein Synthesis in Escherichia coli
• Rabies virus protein synthesis in infected BHK-21 cells.