Protein Synthesis in Newcastle Disease Virus-Infected Chicken Embryo Cells
AUTOR(ES)
Hightower, Lawrence E.
RESUMO
A double-isotopic label difference analysis of polyacrylamide gels has been used to distinguish between cellular and viral protein accumulation in infected cells and to quantify the kinetics of accumulation of viral polypeptides. This technique, coupled with the determination of total radioactive amino acid incorporation in infected cultures, has revealed the following kinetic patterns. Viral polypeptides are first detected in infected cultures 2.0 to 2.5 h postinfection. The rate of accumulation of radioactive amino acids in viral polypeptides increases to a maximum (30 to 35% of the rate of accumulation in uninfected control cultures), whereas the rate of accumulation of radioactive amino acids in host-cell protein decreases to a minimum (20% of the rate of accumulation in uninfected control cultures) by 5 to 6 h postinfection. All of the viral polypeptides detected late in infection are also present at the earlier times, and the major virion structural polypeptides are present in approximately the same (N/G-2, 53K) or slightly increasing (L, G-1, M) relative amounts. One peak area containing a nonstructural glycopeptide with an apparent molecular weight of 66,000 shows significant alterations in rates of accumulation during infection. Inhibition in the rate of radioactive amino acid incorporation into both trichloroacetic acid-soluble and acid-precipitable material during infection has been demonstrated. However, these two inhibition phenomena can be uncoupled temporally by incubating infected cultures at 36 C instead of the usual 40 C, suggesting that they may not be directly related.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=355377Documentos Relacionados
- Thymidine metabolism and DNA synthesis in Newcastle disease virus-infected cells.
- INHIBITION OF RIBONUCLEIC ACID SYNTHESIS IN NEWCASTLE DISEASE VIRUS-INFECTED CELLS BY PUROMYCIN AND 6-AZAURIDINE
- Effect of 5-methylcytidine on virus production in avian sarcoma virus-infected chicken embryo cells.
- Selective inhibition of protein synthesis in virus-infected mammalian cells.
- Control of protein synthesis in Semliki forest virus-infected cells.