Proteomic analisys of bovine yolk sac / Análise proteômica do saco vitelino de bovinos

AUTOR(ES)

Alvaro Carlos Galdos Riveros

DATA DE PUBLICAÇÃO

2009

RESUMO

The yolk sac plays an important role in embryonic development of all mammals. Its function is being studied, proving to be one of the initials of haematopoiesis. In the period when the placenta is not real yet formed during embryonic development, the yolk sac is the main source of nutrition of the embryo, providing a place for the transfer and synthesis of proteins. Proteins are molecules that govern all cellular functions and are from a chemical structurally the most complex biological molecules. The proteomics is the large-scale study of proteins in a complex biological sample. The aim of this work was make the analisys of present proteins in the yolk sac of bovine embryos by 2D-PAGE two-dimensional electrophoresis. The gels obtained from 2D-PAGE of protein samples contained in the yolk sac of 37 days of pregnancy had about 1230 proteins, and gels of samples from 23 days of pregnancy had about 970 proteins. The yolk sac of 23 days showed essential proteins differentially expressed in stages of development, as Hemogen, protein expressed in stage, responsible for controlling the proliferation and differentiation of hematopoietic cells, the protein acetylgalactosamine glycoprotein-N-3-beta-galactosyltransferase-1, involved in the process of angiogenesis, trombopoiese homeostasis and development of the kidney, the protein factor chorion-specific transcription (GCMa) transcription factor necessary for the development of the placenta. The yolk sac of 37 days showed essential proteins differentially expressed in stages of development , as apolipoprotein E (APOE), which mediates the binding, intersignaling and catabolism of lipoprotein particles, and can serve as a link to the receptor to LDL (apo B/E) and specific receptors for apo E in the liver tissue embryogenesisThese proteins are of vital importance to the development of the embryo until the formation of the placenta.

ASSUNTO(S)

yolk sac proteomic analisys embryo bovine análise proteômica two-dimensional electrophoresis eletroforese bidimensional embrião bovinos saco vitelino

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