PRP28, a 'DEAD-box' protein, is required for the first step of mRNA splicing in vitro.
AUTOR(ES)
Strauss, E J
RESUMO
We previously reported the isolation of PRP28, a gene in Saccharomyces cerevisiae whose activity is required for the first step of nuclear mRNA splicing in vivo. Sequence analysis revealed that PRP28 is included in the 'DEAD-box' gene family, members of which are thought to function as ATP-dependent RNA helicases. Genetic interactions led us to suggest that PRP28 is functionally associated with the U4/U5/U6 snRNP. We have now purified the PRP28 protein from S. cerevisiae and demonstrated that it is required for the first step of splicing in vitro. Interestingly, PRP28 is not a stably associated snRNP protein. Strand displacement assays indicate that PRP28 does not exhibit RNA helicase activity, suggesting that an additional factor or factors may be required for its activation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=310295Documentos Relacionados
- Genetic interactions of conserved regions in the DEAD-box protein Prp28p.
- A DEAD-box-family protein is required for nucleocytoplasmic transport of yeast mRNA.
- PRP5: a helicase-like protein required for mRNA splicing in yeast.
- Nuclear PRP20 protein is required for mRNA export.
- Bacterial DNA replication initiation factor priA is related to proteins belonging to the 'DEAD-box' family.
