Purification and Concentration of Influenza Types A and B by Chromatography on Calcium Phosphate
AUTOR(ES)
Lapidus, M.
RESUMO
A rapid chromatographic method for the isolation of types A and B influenza virus from allantoic fluid was described. The adsorbent was prepared from calcium dihydrogen orthophosphate monohydrate [Ca(H2PO4)2·H2O] by alkali treatment. The addition of sodium trimetaphosphate to the influenza-infected allantoic fluid afforded a 67 to 100% viral recovery and a 26 to 43-fold increase in purity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=377730Documentos Relacionados
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