Purification and DNA Synthesis in Cell-Free Extracts: Properties of DNA Polymerase II
AUTOR(ES)
Kornberg, Thomas
RESUMO
The major DNA-synthesizing enzyme present in Pol A1-Escherichia coli (DNA polymerase II) has been purified to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme requires all four deoxynucleoside triphosphates, Mg++, NH4+, and native DNA for maximal activity. The enzyme activity is sensitive to sulfhydryl reagents and is insensitive to anti-DNA polymerase I antiserum. A second DNA-synthesizing enzyme, present in low amounts, has been identified in Pol A1- extracts. The relationship of this enzyme to DNA polymerases I and II is discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=389037Documentos Relacionados
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