Purification and Partial Characterization of Bacillus subtilis Flagellar Hooks1
AUTOR(ES)
Dimmitt, K.
RESUMO
A method for preparing bacterial flagellar hook structures is described. The method involves isolating intact flagella from a mutant which makes thermally labile flagellar filaments and heat-treating them to disaggregate the filament preferentially. The resulting hook preparation can be separated and purified by velocity and isopycnic centrifugation. The purified hooks sediment at a relative S value of 77. On acrylamide gel electrophoresis in sodium dodecyl sulfate, they show one major and a number of minor protein bands. The purified hooks can be used to immunize rabbits, and the resulting antiserum is hook-specific. These results support the notion that hooks are composed of a protein that differs from flagellin.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=247063Documentos Relacionados
- Isolation and characterization of Caulobacter crescentus flagellar hooks.
- Transformation in Bacillus subtilis: purification and partial characterization of a membrane-bound DNA-binding protein.
- Purification and partial characterization of thermostable serine alkaline protease from a newly isolatedBacillus subtilis PE-11
- Partial Purification and Characterization of Dihydrodipicolinic Acid Synthetase from Sporulating Bacillus megaterium1
- Structure of Plant Cell Walls: IX. Purification and Partial Characterization of a Wall-degrading Endo-Arabanase and an Arabinosidase from Bacillus subtilis1