Purification of Hemoglobin from the Actinorhizal Root Nodules of Myrica gale L.
AUTOR(ES)
Pathirana, S. M.
RESUMO
Hemoglobins are generally absent or present in low concentrations in the nodules of actinorhizal plants. An exception is Casuarina, where a hemoglobin occurs at relatively high concentration. However, this plant is unique in that Frankia, the microsymbiont, lacks the vesicles that are normally the site of nitrogen fixation. The present paper shows that a hemoglobin also occurs at high concentrations in Myrica gale L., an actinorhizal plant in which Frankia does form vesicles. Hemoglobin was extracted from root nodules under anaerobic conditions using a buffer containing CO, detergent, and a reducing agent. Carboxyhemoglobin was purified using gel filtration followed by aerobic ion-exchange chromatography. The optical absorption spectra of the oxy-, deoxy-, and carboxyhemoglobins were similar to those of other hemoglobins. The molecular mass of the native hemoglobin estimated by gel filtration was 38,500 D. The molecular mass of the subunits estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was 16,200 D, consistent with the mass of other hemoglobin subunits. Thus, the native hemoglobin is probably a dimer.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=157199Documentos Relacionados
- Factors Affecting the Acetylene to 15N2 Conversion Ratio in Root Nodules of Myrica gale L.
- The Resistance of the Diffusion Barrier in Nodules of Myrica gale L. Changes in Response to Temperature but Not to Partial Pressure of O2.
- Isolation of Frankia Strains from Alder Actinorhizal Root Nodules
- Hydrogenase in actinorhizal root nodules and root nodule homogenates.
- Natural Diversity of Frankia Strains in Actinorhizal Root Nodules from Promiscuous Hosts in the Family Myricaceae