Purification of NADH-Nitrate Reductase by Affinity Chromatography 1
AUTOR(ES)
Solomonson, Larry P.
RESUMO
Assimilatory nitrate reductase (NADH: nitrate oxidoreductase, EC 1.6.6.1) from Chlorella vulgaris has been purified to electrophoretic homogeneity with an overall yield of 60% by a procedure that utilizes blue dextran-agarose as an affinity column. Nitrate reductase binds to blue dextran and is not eluted in the presence of high ionic strength buffer but is rapidly eluted in the presence of μmolar concentrations of NADH.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=541940Documentos Relacionados
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