Pyrimidine-specific chemical reactions useful for DNA sequencing.
AUTOR(ES)
Rubin, C M
RESUMO
Potassium permanganate reacts selectively with thymidine residues in DNA (1) while hydroxylamine hydrochloride at pH 6 specifically attacks cytosine (2). We have adopted these reactions for use with the chemical sequencing method developed by Maxam and Gilbert (3).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=324374Documentos Relacionados
- PYRIMIDINE-SPECIFIC ANTIBODIES WHICH REACT WITH DEOXYRIBONUCLEIC ACID (DNA)*
- Pyrimidine-Specific Carbamyl Phosphate Synthetase in Neurospora crassa
- Pyrimidine-specific cleavage by an endoribonuclease of Saccharomyces cerevisiae.
- Identification of an intracellular pyrimidine-specific endoribonuclease from Bacillus subtilis.
- An improvement in thymine specific chemical DNA sequencing.