Pyrophosphatase is essential for growth of Escherichia coli.
AUTOR(ES)
Chen, J
RESUMO
The ppa gene for inorganic pyrophosphatase is essential for the growth of Escherichia coli. A recombinant with a chromosomal ppa::Kanr lesion and a temperature-sensitive replicon with a ppa+ gene showed a temperature-sensitive growth phenotype, and a mutant with the sole ppa+ gene under control of the lac promoter showed inducer-dependent growth. When the lacp-ppa mutant was subcultured without inducer, the pyrophosphatase level decreased, the PPi level increased, and growth stopped. Cellular PPi reached 16 mM about 6 h after growth arrest without loss of cell viability.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=526883Documentos Relacionados
- The rnh gene is essential for growth of Escherichia coli.
- The ispB gene encoding octaprenyl diphosphate synthase is essential for growth of Escherichia coli.
- Proton motive force is not obligatory for growth of Escherichia coli.
- Methionine aminopeptidase gene of Escherichia coli is essential for cell growth.
- Translation initiation factor IF1 is essential for cell viability in Escherichia coli.