Quantification of arabinitol in serum by selected ion monitoring as a diagnostic technique in invasive candidiasis.
AUTOR(ES)
Roboz, J
RESUMO
D-Arabinitol was identified by mass spectrometry as a metabolite of Candida albicans and Candida tropicalis. For quantification, serum was deproteinized with acetone, the supernatant was evaporated to dryness, the silyl derivative was formed, and a portion was injected into a combined gas chromatograph-mass spectrometer system. Erythritol or 2-deoxy-galactitol was the internal standard. The protonated molecular ions, obtained in chemical ionization with isobutane, were monitored. For 39 normal subjects the mean endogenous arabinitol concentration was 0.52 microgram/ml (standard deviation, +/- 0.34). An increment of 0.2 microgram of arabinitol per ml could be quantified. Of 11 cases with diagnosed invasive candidiasis, 9 had arabinitol levels > 1.2 microgram/ml (8 microM) in the range of 1.2 to 25.0 micrograms/ml; the remaining 2 cases had levels in the normal range. Six cases of diagnosed colonized candidiasis showed normal arabinitol levels.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=273644Documentos Relacionados
- Evaluation of serum arabinitol as a diagnostic test for candidiasis.
- Comparison of serum mannan, arabinitol, and mannose in experimental disseminated candidiasis.
- Simultaneous determination of arabinitol and mannose by gas-liquid chromatography in experimental candidiasis.
- Mannose in body fluids as an indicator of invasive candidiasis.
- Laboratory diagnosis of invasive candidiasis.
