Quantitation of Dam methyltransferase in Escherichia coli.
AUTOR(ES)
Boye, E
RESUMO
An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E. coli cell (doubling time = 30 min) was found to contain about 130 molecules of Dam methyltransferase.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=206566Documentos Relacionados
- Chromosomal replication incompatibility in Dam methyltransferase deficient Escherichia coli cells.
- HpaII methyltransferase is mutagenic in Escherichia coli.
- The oriC unwinding by dam methylation in Escherichia coli.
- Direct role of the Escherichia coli Dam DNA methyltransferase in methylation-directed mismatch repair.
- A second DNA methyltransferase repair enzyme in Escherichia coli.