Quantitative colorimetric microneutralization assay for characterization of adenoviruses.
AUTOR(ES)
Crawford-Miksza, L K
RESUMO
A microneutralization assay that automates the reading and interpretation of viral infectivity and neutralization data was developed for the characterization of adenoviruses (AV). Virus, serum, and cells were added to 96-well plates, incubated for 7 days, and stained with vital stain. The A550 of solubilized dye was read in a plate reader interfaced to a personal computer which analyzed the results. Correlation of A550 values with visual observation of cytopathic effect was extremely good (r = 0.977625). Clinical isolates of 17 AV from 11 patients were characterized by colorimetric neutralization assay. Prototype AV titers tested were comparable to those determined by tube methods. Prototype homotypic antiserum titers were comparable to or greater than those determined by standard tube neutralization.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=263999Documentos Relacionados
- Monoclonal antibody enzyme-linked immunosorbent assay for specific identification and typing of subgroup F adenoviruses.
- Genome typing of mouse adenoviruses.
- Simplified restriction endonuclease method for typing and subtyping adenoviruses.
- Rapid presumptive recognition of diarrhea-associated adenoviruses.
- Sequence homology between avian and human adenoviruses.