Quantitative requirements for exponential growth of Alcaligenes eutrophus.
AUTOR(ES)
Repaske, R
RESUMO
Quantitative nutrient requirements for unrestricted autotrophic growth of Alcaligenes eutrophus were determined. Minimum saturating concentrations of Mg2+, SO42-, PO43-, Fe3+, and Na2+ for an optical density increase of 2 were 10(-4) M 8 X10(-5) M, 5 X 10(-4) to 6 X 10(-4) M, 10(-5) M, and 10(-7) to 2 X 10(-7) M, respectively. Trace metal requirements for cobalt, chromium, and copper were also demonstrated, but minimum concentrations could not be determined because other reagents contributed a high background of these metals. Under certain conditions an apparent response to zinc was observed, although other experiments suggest the zinc salt contained another metal that was required for growth. Poly-beta-hydroxybutyrate biosynthesis was shown to be initiated by a magnesium or sulfate deficiency as well as by a nitrogen or phosphate deficiency.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=170311Documentos Relacionados
- Dense autotrophic cultures of Alcaligenes eutrophus.
- Nickel requirement for active hydrogenase formation in Alcaligenes eutrophus.
- Identification of a novel gene, aut, involved in autotrophic growth of Alcaligenes eutrophus.
- Chromosomal and plasmid locations for phosphoribulokinase genes in Alcaligenes eutrophus.
- Nickel in the catalytically active hydrogenase of Alcaligenes eutrophus.
