Randomly picked cosmid clones overlap the pyrB and oriC gap in the physical map of the E. coli chromosome.
AUTOR(ES)
Knott, V
RESUMO
Sets of overlapping cosmid clones generated by random sampling and fingerprinting methods complement data at pyrB (96.5') and oriC (84') in the published physical map of E. coli. A new cloning strategy using sheared DNA, and a low copy, inducible cosmid vector were used in order to reduce bias in libraries, in conjunction with micro-methods for preparing cosmid DNA from a large number of clones. Our results are relevant to the design of the best approach to the physical mapping of large genomes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=336392Documentos Relacionados
- Physical distance between the site of type II DNA binding to the membrane and oriC on the Bacillus subtilis 168 chromosome.
- The 245 base-pair oriC sequence of the E. coli chromosome directs bidirectional replication at an adjacent region.
- Absence of a direct role for RNase HI in initiation of DNA replication at the oriC site on the Escherichia coli chromosome.
- Site-directed mutagenesis of the Escherichia coli chromosome near oriC: identification and characterization of asnC, a regulatory element in E. coli asparagine metabolism.
- Physical map of the tolC-htrP region of the Escherichia coli chromosome.