Rapid, colorimetric test for the determination of hippurate hydrolysis by group B Streptococcus.
AUTOR(ES)
Edberg, S C
RESUMO
A colorimetric test for the determination of hippurate hydrolysis was developed. Brain heart infusion broth made with 1% sodium hippurate served as the test medium. Hydrolysis was determined by the addition of two chemical developers, M (rhodamine B) and A (uranium acetate). A dark pink color indicated hydrolysis; no color change indicated no hydrolysis. The method was efficacious in either rapid or overnight incubation. One hundred twenty-five strains of group B, 44 strains of group A, 15 strains of group C, and 10 strains of group G Streptococcus were tested. By using the Lancefield method as the standard, there was 100% agreement with both the colorimetric and ferric chloride tests for hippurate hydrolysis, and 96% agreement with the CAMP test.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=274225Documentos Relacionados
- Rapid Hippurate Hydrolysis Method for Presumptive Identification of Group B Streptococci
- Evaluation of the rapid hippurate hydrolysis test with enterococcal group D streptococci.
- Evaluation of the Rapid Hippurate Hydrolysis Test with Enterococcal Group D Streptococci
- Endocarditis caused by nonhemolytic group B streptococcus.
- Micro-indirect hemagglutination test for detection of antibodies to the Ibc protein of group B Streptococcus.
