Rapid method for processing soil samples for polymerase chain reaction amplification of specific gene sequences.
AUTOR(ES)
Pillai, S D
RESUMO
Bacterial cells can be differentially separated from soil colloids on the basis of their buoyant densities. By using this principle, a modified sucrose gradient centrifugation protocol has been developed for separating bacterial cells from most of the soil colloids. Since the bacterial cell suspension still contained some colloidal soil particles, which inhibited polymerase chain reaction amplification, a new "double" polymerase chain reaction method of analysis was adopted for amplification of Tn5-specific gene sequences. This new protocol allowed rapid detection of small numbers (1 to 10 CFU/g) of bacterial cells present in soil samples.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=183564Documentos Relacionados
- Combined subtraction hybridization and polymerase chain reaction amplification procedure for isolation of strain-specific Rhizobium DNA sequences.
- Alu polymerase chain reaction: a method for rapid isolation of human-specific sequences from complex DNA sources.
- Polymerase Chain Reaction—A Novel Method for Analyzing Specific DNA Sequences
- Rapid plasmid insert amplification with polymerase chain reaction.
- Enzymatic amplification of human cytomegalovirus sequences by polymerase chain reaction.