Rapid PCR amplification of minimal enediyne polyketide synthase cassettes leads to a predictive familial classification model

AUTOR(ES)

Liu, Wen

FONTE

National Academy of Sciences

RESUMO

A universal PCR method for the rapid amplification of minimal enediyne polyketide synthase (PKS) genes and the application of this methodology to clone remaining prototypical genes from producers of structurally determined enediynes in both family types are presented. A phylogenetic analysis of the new pool of bona fide enediyne PKS genes, consisting of three from 9-membered producers (neocarzinostatin, C1027, and maduropeptin) and three from 10-membered producers (calicheamicin, dynemicin, and esperamicin), reveals a clear genotypic distinction between the two structural families from which to form a predictive model. The results from this study support the postulation that the minimal enediyne PKS helps define the structural divergence of the enediyne core and provides the key tools for generating enediyne hybrid genes/molecular scaffolds; by using the model, a classification is also provided for the unknown enediyne PKS genes previously identified via genome scanning.

Documentos Relacionados

• Engineered biosynthesis of novel polyketides: influence of a downstream enzyme on the catalytic specificity of a minimal aromatic polyketide synthase.
• Ectopic expression of the minimal whiE polyketide synthase generates a library of aromatic polyketides of diverse sizes and shapes
• Rapid diagnosis of poliovirus infection by PCR amplification.
• Rapid extraction of fungal DNA for PCR amplification.
• Type I polyketide synthase requiring a discrete acyltransferase for polyketide biosynthesis