Rapid quantitative microenzyme-linked immunosorbent assay for tetanus antibodies.
AUTOR(ES)
Sedgwick, A K
RESUMO
A rapid quantitative microenzyme-linked immunosorbent assay for the detection of immunoglobulin G antibodies to tetanus toxoid is described. The assay is performed in 2.5 h and detects antibody levels of between 0.1 and 6 U/ml. Quantitation of the antibody concentration is unique in that multiple serum dilutions are tested on a single patient, the results are plotted as an antibody dilution curve, and the area under the curve is integrated for a final concentration. Tests for immune status on sera from 77 individuals demonstrated tetanus antibody concentrations of greater than or equal to 0.1 U/ml in 92% of a pediatric group and 62% of an adult group. The microenzyme-linked immunosorbent assay procedure is reproducible and is useful for the determination of immune status and immunization response.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=270751Documentos Relacionados
- Microenzyme-linked immunosorbent assay for the detection of specific IgM antibodies in human syphilis.
- Microenzyme-Linked Immunosorbent Assay for Detection of Immunoglobulin G and Immunoglobulin M Antibodies to Legionella pneumophila
- Evaluation of the microenzyme-linked immunosorbent assay with Treponema pallidum antigen.
- Detection of antibody to staphylococcal lipoteichoic acid with a microenzyme-linked immunosorbent assay.
- Rapid enzyme-linked immunosorbent assay (ELISA) for Aspergillus fumigatus antibodies.