Rapid, solid-phase based automated analysis of chromatin structure and transcription factor occupancy in living eukaryotic cells

AUTOR(ES)

Ingram, Richard

FONTE

Oxford University Press

RESUMO

Transcription factors, chromatin components and chromatin modification activities are involved in many diseases including cancer. However, the means by which alterations in these factors influence the epigenotype of specific cell types is poorly understood. One problem that limits progress is that regulatory regions of eukaryotic genes sometimes extend over large regions of DNA. To improve chromatin structure–function analysis over such large regions, we have developed an automated, relatively simple procedure that uses magnetic beads and a capillary sequencer for ligation-mediated-PCR (LM-PCR). We show that the procedure can be used for the rapid examination of chromatin fine-structure, nucleosome positioning as well as changes in transcription factor binding-site occupancy during cellular differentiation.

Documentos Relacionados

• Solid-phase extractions in flow analysis
• Rapid Solid-Phase Radioimmunoassay for Staphylococcal Enterotoxin A
• Rapid synthesis of oligodeoxyribonucleotides: a new solid-phase method.
• Matrix solid-phase dispersion versus ultrasound assisted extraction with solid-phase extraction in the HPLC analysis of furanocoumarins from fruits of Archangelica officinalis Hoffm
• Rapid Detection and Enumeration of Naegleria fowleri in Surface Waters by Solid-Phase Cytometry