Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification

AUTOR(ES)

Silva, Gildo Almeida da, Bernardi, Taís Letícia, Schaker, Patrícia Dayane Carvalho, Menegotto, Morgana, Valente, Patricia

FONTE

Brazilian Archives of Biology and Technology

DATA DE PUBLICAÇÃO

2012-04

RESUMO

The purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade PCR-product. A specific DNA fragment of the 18S region of Dekkera bruxellensis and Saccharomyces cerevisiae was chosen. The described boiling and freeze-thawing protocols generated the PCR-grade product preparations and could be used to process many samples. The amplification of the fragments could be observed after 30 and 35 cycles. These processes of extraction without using any kind of chemical reagents, especial water, and purification procedures proved to be efficient, reproducible, simple, fast, and inexpensive.

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