Recombination walking: genetic selection of clones from pooled libraries of yeast artificial chromosomes by homologous recombination.

AUTOR(ES)

Miller, A M

RESUMO

Recombination walking is based on the genetic selection of specific human clones from a yeast artificial chromosome (YAC) library by homologous recombination. The desired clone is selected from a pooled (unordered) YAC library, eliminating labor-intensive steps typically used in organizing and maintaining ordered YAC libraries. Recombination walking represents an efficient approach to library screening and is well suited for chromosome-walking approaches to the isolation of genes associated with common diseases.

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