Reconstitution of a Chemically Excitable Membrane
AUTOR(ES)
Hazelbauer, Gerald L.
RESUMO
Membrane fragments rich in cholinergic (nicotinic) receptor sites, purified from homogenates of Torpedo marmorata electric organ, are dissolved in high concentrations of Na cholate and Tris buffer in the presence of Na ethylenediaminetetraacetate, without loss of the ability to bind Naja nigricollis [3H]α-toxin. After extensive dialysis to remove cholate, the solution is supplemented with crude lipids extracted from native membrane fragments and MgCl2-CaCl2 added dropwise to the mixture as a concentrated solution. The reconstituted membranes obtained retain 22Na+. Release of Na from these microsacs increases in the presence of carbamylcholine and the α-toxin from N. nigricollis blocks this effect.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=388253Documentos Relacionados
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