Redistribution of Tritium during Germination of Grain Harvested from myo-[2-3H]Inositol- and scyllo-[R-3H]Inositol-Labeled Wheat 1

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RESUMO

Wheat kernels from myo-[2-3H]inositol- or scyllo-[R-3H]inositol-labeled plants (Sasaki and Loewus 1980 Plant Physiol 66: 740-745) were used to study redistribution of 3H into growing regions during germination. Most of the labeled 1-α-galactinol (or the analogous scyllo-inositol galactoside) was hydrolyzed within 1 day. Water-soluble phytate was dephosphorylated within 3 days. A large reserve of bound phytate continued to release myo-inositol over several days. Translocation of free myo-inositol to growing regions provided substrate for the myo-inositol oxidation pathway and incorporation of 3H into new cell wall polysaccharides.

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