Regulated Expression of a Highly Conserved Regulatory Gene Cluster Is Necessary for Controlling Photosynthesis Gene Expression in Response to Anaerobiosis in Rhodobacter capsulatus
AUTOR(ES)
Du, Shouying
FONTE
American Society for Microbiology
RESUMO
We utilized primer extension analysis to demonstrate that the divergently transcribed regB and senC-regA-hvrA transcripts contain stable 5′ ends 43 nucleotides apart within the regB-senC intergenic region. DNA sequence analysis indicates that this region contains two divergent promoters with overlapping ς70 type −35 and −10 promoter recognition sequences. In vivo analysis of expression patterns of regB::lacZ and senC-regA-hvrA::lacZ reporter gene fusions demonstrates that the regB and senC-regA-hvrA transcripts are both negatively regulated by the phosphorylated form of the global response regulator RegA. DNase I protection assays with a constitutively active variant of RegA indicate that RegA binds between regB and senC overlapping −10 and −35 promoter recognition sequences. Two mutations were also isolated in a regB-deficient background that increased expression of the senC-regA-hvrA operon 10- and 5-fold, respectively. As a consequence of increased RegA expression, these mutants exhibited elevated aerobic and anaerobic photosynthesis (puf) gene expression, even in the absence of the sensor kinase RegB. These results indicate that autoregulation by RegA is a factor contributing to the maintenance of an optimal low level of RegA expression that allows responsiveness to activation by phosphorylation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=93936Documentos Relacionados
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