Regulation of dihydrodipicolinate synthase during growth and sporulation of Bacillus cereus.
AUTOR(ES)
Hoganson, D A
RESUMO
A four- to sixfold increase in specific activity of dihydrodipicolinic acid synthase was observed during sporulation of Bacillus cereus. The enzyme from cells harvested before and after the increase in specific activity appeared to be very similar as judged by pH optima, heat denaturation kinetics, apparent Michaelis constants, chromatography on diethylaminoethyl-cellulose and Sephadex G-200, and polyacrylamide gel electrophoresis. Studies with various combinations of amino acids and one of the enzyme substrates, pyruvate, failed to give evidence for control of the enzyme by activation, inhibition, repression, induction, or stabilization. Omission of calcium from the sporulation medium had no significant effect on the specific activity pattern of the enzyme as a function of age of culture.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=236046Documentos Relacionados
- Bacterial Sporulation and Regulation of Dihydrodipicolinate Synthase in Ribonucleic Acid Polymerase Mutants of Bacillus subtilis
- Lipid Composition of Bacillus cereus During Growth and Sporulation
- BIOCHEMICAL CHANGES OCCURRING DURING GROWTH AND SPORULATION OF BACILLUS CEREUS12
- Regulation of dihydrodipicolinate synthase and aspartate kinase in Bacillus subtilis.
- EFFECT OF pH ON INTERMEDIATES PRODUCED DURING GROWTH AND SPORULATION OF BACILLUS CEREUS