Regulation of Glycolytic Oscillations by Mitochondrial and Plasma Membrane H+-ATPases

AUTOR(ES)

Olsen, Lars Folke

FONTE

The Biophysical Society

RESUMO

We investigated the coupling between glycolytic and mitochondrial membrane potential oscillations in Saccharomyces cerevisiae under semianaerobic conditions. Glycolysis was measured as NADH autofluorescence, and mitochondrial membrane potential was measured using the fluorescent dye 3,3′-diethyloxacarbocyanine iodide. The responses of glycolytic and membrane potential oscillations to a number of inhibitors of glycolysis, mitochondrial electron flow, and mitochondrial and plasma membrane H+-ATPase were investigated. Furthermore, the glycolytic flux was determined as the rate of production of ethanol in a number of different situations (changing pH or the presence and absence of inhibitors). Finally, the intracellular pH was determined and shown to oscillate. The results support earlier work suggesting that the coupling between glycolysis and mitochondrial membrane potential is mediated by the ADP/ATP antiporter and the mitochondrial F0F1-ATPase. The results further suggest that ATP hydrolysis, through the action of the mitochondrial F0F1-ATPase and plasma membrane H+-ATPase, are important in regulating these oscillations. We conclude that it is glycolysis that drives the oscillations in mitochondrial membrane potential.

Documentos Relacionados

• Nongenomic stimulation of vacuolar H+-ATPases in intercalated renal tubule cells by aldosterone
• The Two Major Types of Plant Plasma Membrane H+-ATPases Show Different Enzymatic Properties and Confer Differential pH Sensitivity of Yeast Growth1
• Ca2+ accumulation into acidic organelles mediated by Ca2+- and vacuolar H+-ATPases in human platelets
• Modulacion of H+-ATPases activity and the cell membranes unity in two bananas genotip (Musa spp) submited to salt stress.
• Regulation of fibroblast mitochondrial 27-hydroxycholesterol production by active plasma membrane cholesterol