Regulation of growth hormone messenger RNA by thyroid and glucocorticoid hormones.
AUTOR(ES)
Martial, J A
RESUMO
Thyroid and glucocorticoid hormones stimulate growth hormone synthesis in cultured rat pituitary cells (GC). We have compared changes in growth hormone production and mRNA in these cells. Triiodothyronine (10 nM) and dexamethasone (1 micron) stimulated increases in growth hormone production by 2.5- and 3.8-fold, respectively. There were corresponding increases in the capacity of RNA from hormone-treated cells to direct synthesis of pregrowth hormone in a wheat germ cell-free translation system, suggesting hormone-regulated increases in growth hormone mRNA. Hormone-induced changes in mRNA were also demonstrated by determining the kinetics of hybridization of a cDNA probe prepared from RNA enriched (about 70%) for growth hormone translational activity with RNA from control and hormone-treated cells. These results suggest that thyroid and glucocorticoid hormones can regulate growth hormone production by influencing the levels of its mRNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=431013Documentos Relacionados
- Regulation of growth hormone gene expression: synergistic effects of thyroid and glucocorticoid hormones.
- Regulation of rat luteinizing hormone subunit messenger ribonucleic acids by gonadal steroid hormones.
- Glucocorticoid and thyroid hormones transcriptionally regulate growth hormone gene expression.
- Thyroid and glucocorticoid hormones synergistically control growth hormone mRNA in cultured GH1 cells.
- Dexamethasone regulation of glycosaminoglycan synthesis in cultured human skin fibroblasts. Similar effects of glucocorticoid and thyroid hormones.