Regulation of H+ Excretion 1: EFFECTS OF OSMOTIC SHOCK

AUTOR(ES)

Rubinstein, Bernard

RESUMO

Osmotic shock, a 15-minute plasmolysis followed by a 15-minute rehydration in the cold, is a nondestructive technique which inhibits fusicoccin-stimulated H+ excretion from oat mesophyll cells (Avena sativa L.). Osmotic shock also causes a loss of intracellular solutes and stimulates H+ uptake, but osmoregulation can still occur, and enhanced H+ uptake is observed only at low external pH. It is concluded that osmotic shock interferes directly with the excretion of H+ rather than affecting only H+ or counter ion uptake.

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