Regulation of Histidyl-Transfer Ribonucleic Acid Synthetase Formation in a Histidyl-Transfer Ribonucleic Acid Synthetase Mutant of Salmonella typhimurium
AUTOR(ES)
McGinnis, E.
RESUMO
Control of formation of the histidyl-transfer ribonucleic acid (tRNA) synthetase with an increased Km for histidine was studied in a hisS mutant of Salmonella typhimurium. Histidine restriction of both the hisS and hisS+ strains resulted in a derepression of synthesis of histidyl-tRNA synthetase. When grown in a concentration less than the Km (100 μg/ml) of l-histidine, the hisS mutant maintained a higher level of histidyl-tRNA synthetase than the hisS+ strain. Addition of excess amounts of l-histidine to the growth medium of the hisS mutant culture grown with 100 μg of l-histidine per ml resulted in a repression of histidyl-tRNA synthetase formation to equal that of the hisS+ strain grown in 100 μg of l-histidine per ml. These data confirm previous findings that histidine tRNA is involved in the repression of synthesis of histidyl-tRNA synthetase.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=251347Documentos Relacionados
- Role of Histidine Transfer Ribonucleic Acid in Regulation of Synthesis of Histidyl-Transfer Ribonucleic Acid Synthetase of Salmonella typhimurium
- Histidyl-Transfer Ribonucleic Acid Synthetase in Positive Control of the Histidine Operon in Salmonella typhimurium1
- First Enzyme of Histidine Biosynthesis and Repression Control of Histidyl-Transfer Ribonucleic Acid Synthetase of Salmonella typhimurium
- Defective in vitro binding of histidyl-transfer ribonucleic acid to feedback resistant phosphoribosyl transferase of Salmonella typhimurium
- Histidyl-Transfer Ribonucleic Acid Synthetase Mutants Requiring a High Internal Pool of Histidine for Growth
