Regulation of Lymphocyte Responses In Vitro: Potentiation and Inhibition of Rat Lymphocyte Responses to Antigen and Mitogens by Cytochalasin B*

AUTOR(ES)

Yoshinaga, Masaru

RESUMO

Cytochalasin B, at concentration between 0.02 and 0.2 μg/ml, was slightly stimulatory to lymph-node cells from normal rats and greatly potentiated their response to phytohemagglutinin and low concentrations of concanavalin A (mitogens for thymus-derived lymphocytes); it also potentiated the response of thymocytes to phytohemagglutinin. The response of lymphnode cells to lipopolysaccharide endotoxin (a mitogen for thymus-independent lymphocytes) was also enhanced, but only at concentrations in the usual inhibitory range, possibly by inhibition of a “suppressor T-cell” response. Sensitized lymphocytes responding to antigen were not stimulated at all, except at a very high cell density, where inhibition of a “suppressor cell” response was also considered likely. At concentrations of 5-10 μg/ml or higher, cytochalasin B inhibited all responses tested.

Documentos Relacionados

• Reduced lymphocyte responses to mitogens in natural and experimental trichomoniasis.
• Rat oocyte maturation in vitro: Relief of cyclic AMP inhibition by gonadotropins
• Inhibition of group B arbovirus antigen production and replication in cells enucleated with cytochalasin B.
• Effects of electromagnetic stimuli on bone and bone cells in vitro: Inhibition of responses to parathyroid hormone by low-energy low-frequency fields
• Liposomal amphotericin B inhibits in vitro T-lymphocyte response to antigen.