Regulation of murine class I genes by interferons is controlled by regions located both 5' and 3' to the transcription initiation site.

AUTOR(ES)

Korber, B

RESUMO

Interferons regulate the expression of a large number of mammalian genes, including the major histocompatibility antigen genes. To investigate the mechanisms involved in interferon action, we have analyzed the ability of murine H-2Ld and H-2Dd DNA sequences to control the responses to interferon. The results indicate that interferon regulation of class I gene expression is complex and involves at least two mechanisms that are dependent on class I sequences located upstream and downstream to the transcription initiation site. In transfected mouse L cells, both of these regions are required for full enhancement of class I gene expression, with the major portion of the response controlled by the sequences located 3' to the transcription initiation site. The fine-mapping analysis of the 5' region-encoded response also suggests that recombinant alpha and gamma interferons may exert their effects on class I gene expression by using different cis-acting regulatory sequences.

Documentos Relacionados

• Pausing of reverse transcriptase on retroviral RNA templates is influenced by secondary structures both 5' and 3' of the catalytic site.
• Specific inhibition of aphthovirus infection by RNAs transcribed from both the 5' and the 3' noncoding regions.
• c-mos expression in mouse oocytes is controlled by initiator-related sequences immediately downstream of the transcription initiation site.
• Negative regulation of the 5' long terminal repeat (LTR) by the 3' LTR in the murine proviral genome.
• Interferons increase transcription of a major histocompatibility class I gene via a 5' interferon consensus sequence.