Regulation of polyomavirus transcription by large tumor antigen.
AUTOR(ES)
Farmerie, W G
RESUMO
We have analyzed the regulation of viral transcription by the large tumor antigen in cells infected by several viable deletion and insertion mutants of polyomavirus. We find that deletion of the early promoter "TATA box" and associated large tumor antigen binding site has only a small effect on the balance of early and late mRNAs. Furthermore, transcription of a polyomavirus containing a heterologous adenovirus promoter in place of the normal TATA box and cap sites is regulated by the large tumor antigen. We conclude that repression of polyomavirus early transcription cannot occur simply by binding of the large tumor antigen to DNA sequences at the site of transcription initiation and must involve the interaction of the large tumor antigen binding at other sites.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=392047Documentos Relacionados
- Cyclin-dependent kinase regulation of the replication functions of polyomavirus large T antigen.
- Polyomavirus tumor induction in mice: effects of polymorphisms of VP1 and large T antigen.
- Localization of the phosphorylations of polyomavirus large T antigen.
- Mapping of phosphorylation sites in polyomavirus large T antigen.
- Regulation of simian virus 40 early transcription in vitro by a purified tumor antigen.