Regulation of synthesis of hepatic fatty acid synthetase: polysomal translation in a cell-free system.
AUTOR(ES)
Strauss, A W
RESUMO
Polysomes were isolated from livers of rats fed various diets and were translated in a protein-synthesizing system derived from cultured Chang liver cells. One of the labeled products was identified as complete subunit(s) of fatty acid synthetase by indirect immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the solubilized immunoprecipitate. The relative amounts of fatty acid synthetase synthesized by polysomes from livers of rats fed a normal diet, starved rats, and rats starved and refed a fat-free diet for 16 hr were 1, 0.1, and 10, respectively. Induction of synthesis of fatty acid synthetase after fat-free refeeding of starved rats began by 2 hr (3-fold increase over starved animals), was increasing rapidly by 5 hr (19-fold over starved animals), and reached a high level by 16 hr (95-fold over starved and 10-fold over normally fed).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=388722Documentos Relacionados
- Translation of rat liver fatty acid synthetase mRNA in a cell-free system derived from wheat germ
- Folding of firefly luciferase during translation in a cell-free system.
- Complete translation of poliovirus RNA in a eukaryotic cell-free system.
- De novo synthesis of myosin in a cell-free system.
- Regulation of synthesis of hepatic fatty acid synthetase: binding of fatty acid synthetase antibodies to polysomes.