Regulation of the amplification C3 convertase of human complement by an inhibitory protein isolated from human erythrocyte membrane
AUTOR(ES)
Fearon, Douglas T.
RESUMO
An activity that is inhibitory to the properdin-stabilized amplification C3 convertase (C3b,Bb,P) was solubilized from human erythrocyte (Ehu) membranes by Nonidet P-40 and purified to homogeneity. The inhibitory membrane glycoprotein had an apparent Mr of 1-1.2×106 on gel filtration in the presence of Nonidet P-40. On sodium dodecyl sulfate/polyacrylamide gel electrophoresis it presented a single stained band with an apparent Mr of 205,000, with or without prior reduction of disulfides. The inhibitory protein of the Ehu membrane produced a dose-related, first-order decay of C3b,Bb,P function on sheep erythrocytes (Es) and released 125I-labeled Bb from these sites, indicating a mechanism of inhibition by decay-dissociation of the amplification C3 convertase. The 50% inhibitory dose of the Ehu membrane protein was not altered by removal of sialic acid from the Es bearing C3b,Bb,P sites. Ehu membrane protein also serves as a cofactor for C3b inactivator-induced cleavage of the α polypeptide chain of C3b. Thus, the inhibitory membrane protein can abrogate the activity of amplification convertase sites that have formed and also can prevent generation of such sites by augmenting irreversible inactivation of C3b.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=411753Documentos Relacionados
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