Regulation of the iron uptake system in Vibrio anguillarum: evidence for a cooperative effect between two transcriptional activators.
AUTOR(ES)
Salinas, P C
RESUMO
We have identified a 110-kDa polypeptide that has a trans-acting regulatory activity on the expression of the pJM1 plasmid iron-uptake genes in Vibrio anguillarum. This protein is encoded by the angR gene and maps in a 3.6-kilobase-pair pJM1 DNA region located downstream of the iron transport genes. Full expression of this gene occurs under iron-limiting conditions and requires a 2.9-kilobase-pair upstream region in cis that maps within the coding region of the OM2 outer membrane protein, essential for the transport of iron into the cell cytosol. Determination of the siderophore anguibactin levels as well as analysis of specific transcripts for anguibactin biosynthetic genes demonstrated that AngR and another transcriptional activator, Taf, regulate in a synergistic fashion the level of anguibactin production by activation of transcription of the anguibactin biosynthetic genes under iron-limiting conditions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=287171Documentos Relacionados
- Chromosome-mediated iron uptake system in pathogenic strains of Vibrio anguillarum.
- Iron uptake system medicated by Vibrio anguillarum plasmid pJM1.
- Distinct TFIID complexes mediate the effect of different transcriptional activators.
- Characterization of the angR Gene of Vibrio anguillarum: Essential Role in Virulence
- In vivo footprinting and functional analysis of the human c-sis/PDGF B gene promoter provides evidence for two binding sites for transcriptional activators.