Regulation of transcription by H1 phosphorylation in Tetrahymena is position independent and requires clustered sites
AUTOR(ES)
Dou, Yali
FONTE
The National Academy of Sciences
RESUMO
In Tetrahymena cells, constitutive phosphorylation of histone H1 phenocopies the loss of H1 from chromatin. Regulation of transcription by H1 phosphorylation is achieved by altering the overall charges of a small domain. Here, we further explore the electrostatic properties of this domain and the mechanism by which it regulates transcription. We demonstrate that the regulatory effect of the clustered charges does not require any long-range interaction and is position independent. However, when the same number of charges was dispersed throughout the H1 molecule, the effect became undetectable. The results are explained by a nucleation-propagation model and provide in vivo evidence that the synergy of the clustered positive charges plays a role in histone function and gene regulation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=122916Documentos Relacionados
- Methylation of adenine in the nuclear DNA of Tetrahymena is internucleosomal and independent of histone H1
- Phosphorylation at clustered -Ser-Pro-X-Lys/Arg- motifs in sperm-specific histones H1 and H2B.
- The Dynamic Mobility of Histone H1 Is Regulated by Cyclin/CDK Phosphorylation
- Temporal Activation of the Sea Urchin Late H1 Gene Requires Stage-Specific Phosphorylation of the Embryonic Transcription Factor SSAP
- Endocytosis and recycling of subunit H1 of the asialoglycoprotein receptor is independent of oligomerization with H2.