Regulatory effect of monocytes on T cell proliferative responses to oral microbial antigens.

AUTOR(ES)

Stashenko, P

RESUMO

Mononuclear cell preparations isolated by Ficoll-Hypaque centrifugation from human peripheral blood were found to vary considerably in the number of monocytes they contained (mean, 20.3%; range, 13 to 33%). The regulatory role of monocytes in T cell proliferative responses to sonic extracts of a panel of oral microorganisms was therefore investigated. T cells were fractionated by anti-immunoglobulin chromatography and depleted of monocytes by treatment with a monoclonal anti-human Ia-like (DR locus antigen) antibody and complement. Purified populations of monocytes were obtained by extensive adherence procedures. The resultant cell populations were greater than 95% pure, as judged by indirect immunofluorescence on a fluorescence-activated cell sorter. Monocyte-depleted T cells failed to respond by proliferation to the nonoral antigen tetanus toxoid, as well as to any oral microorganism, but retained responsiveness to phytohemagglutinin. Readdition of monocytes in final concentrations of from 5 to 15% resulted in the restoration of maximal T cell proliferation. Monocytes in greater numbers suppressed T cell responses to all sonic extracts tested.

Documentos Relacionados

• Human T cell responses to dengue virus antigens. Proliferative responses and interferon gamma production.
• Shipment Impairs Lymphocyte Proliferative Responses to Microbial Antigens
• Human cell-mediated immune responses to chlamydial antigens.
• Effect of malnutrition in Ecuadorian children on titers of serum antibodies to various microbial antigens.
• Single-cell studies on hapten-specific B cells: response to T-cell-dependent antigens.