Relaxation of supercoiled plasmid DNA by oxidative stresses in Escherichia coli.
AUTOR(ES)
Horiuchi, H
RESUMO
The relaxation of plasmid DNA was observed after the visible light irradiation of Escherichia coli AB1157 harboring plasmid pBR322 or some other plasmids in the presence of a photosensitizing dye, such as toluidine blue or acridine orange, and molecular oxygen. Treatment of the cells with hydroperoxides, such as tert-butyl hydroperoxide, cumene hydroperoxide, and hydrogen peroxide, also caused the plasmid DNA relaxation in vivo. Relaxation was not observed in these treatments of purified pBR322 DNA in vitro. Plasmid DNA relaxation was also detected after near-UV irradiation. Far-UV irradiation did not induce such relaxation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=215811Documentos Relacionados
- Properties of the relaxation complex of supercoiled deoxyribonucleic acid and protein of R plasmid NR1 in Escherichia coli.
- Positively supercoiled plasmid DNA is produced by treatment of Escherichia coli with DNA gyrase inhibitors.
- Changes in the linking number of supercoiled DNA accompany growth transitions in Escherichia coli.
- Preferential inhibition of plasmid replication in vivo by altered DNA gyrase activity in Escherichia coli.
- Drug-induced relaxation of supercoiled plasmid DNA in Bacillus subtilis and induction of the SOS response.
