Removal of impurities from transcription factor preparations that alter their DNA-binding properties
AUTOR(ES)
Sun, Liping
FONTE
Oxford University Press
RESUMO
Biochemical studies of transcriptional activators are important for understanding their detailed mechanism of action. Such experiments generally employ chimeric constructs comprised of fused DNA- binding and activation domains that are expressed in, and purified from, Escherichia coli, since full-length activators are usually difficult to express. We report here that such preparations contain chaperone impurities that affect the DNA-binding properties of the activator, for example sharply reducing the half-life of the protein–DNA complex. A simple method to remove these troublesome contaminants is described.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=134261Documentos Relacionados
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