Repair of Single-Strand Deoxyribonucleic Acid Breaks in Ultraviolet Light-Irradiated Haemophilus influenzae
AUTOR(ES)
Kantor, George J.
RESUMO
The wild-type strain and mutants of Haemophilus influenzae, sensitive or resistant to ultraviolet light (UV) as defined by colony-forming ability, were examined for their ability to perform the incision and rejoining steps of the deoxyribonucleic acid (DNA) dark repair process. Although UV-induced pyrimidine dimers are excised by the wild-type Rd and a resistant mutant BC200, the expected single-strand DNA breaks could not be detected on alkaline sucrose gradients. Repair of the gap resulting from excision must be rapid when experimental conditions described by us are employed. Single-strand DNA breaks were not detected in a UV-irradiated sensitive mutant (BC100) incapable of excising pyrimidine dimers, indicating that this mutant may be defective in a dimer-recognizing endonuclease. No single-strand DNA breaks were detected in a lysogen BC100(HP1c1) irradiated with a UV dose large enough to induce phage development in 80% of the cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=251687Documentos Relacionados
- Single-strand regions in the deoxyribonucleic acid of competent Haemophilus influenzae.
- Direct Enzymatic Repair of Deoxyribonucleic Acid Single-Strand Breaks in Dormant Spores
- Repair of hydrogen peroxide-induced single-strand breaks in Escherichia coli deoxyribonucleic acid.
- Repair of x-ray-induced deoxyribonucleic acid single-strand breaks in xth mutants of Escherichia coli.
- Repair of Ultraviolet-Irradiated Transforming Deoxyribonucleic Acid in Haemophilus influenzae
